Review



mouse ano2 gene  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    OriGene mouse ano2 gene
    A-C) YFP Fluorescence measurement showing inhibition of mouse <t>ANO2</t> activity by Ani9, T16A inh -A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).
    Mouse Ano2 Gene, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ano2+gene/Ano2+Mouse+qPCR+Template+Standard/pmc04878759-34-22-15
    Average 90 stars, based on 1 article reviews
    mouse ano2 gene - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2"

    Article Title: Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0155771

    A-C) YFP Fluorescence measurement showing inhibition of mouse ANO2 activity by Ani9, T16A inh -A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).
    Figure Legend Snippet: A-C) YFP Fluorescence measurement showing inhibition of mouse ANO2 activity by Ani9, T16A inh -A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).

    Techniques Used: Fluorescence, Inhibition, Activity Assay, Expressing, Activation Assay, Concentration Assay

    Related Articles

    Expressing:

    Article Title: Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2
    Article Snippet: .. ANO2 expressing FRT cells were obtained by stable transfection of FRT cells with a pCMV6-ANO2 (Origene Technologies Inc), plasmid which expresses the mouse ANO2 gene and a pcDNA3.1-YFP-F46L/H148Q/I152L plasmid which expresses halide sensor YFP gene. ..

    Stable Transfection:

    Article Title: Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2
    Article Snippet: .. ANO2 expressing FRT cells were obtained by stable transfection of FRT cells with a pCMV6-ANO2 (Origene Technologies Inc), plasmid which expresses the mouse ANO2 gene and a pcDNA3.1-YFP-F46L/H148Q/I152L plasmid which expresses halide sensor YFP gene. ..

    Plasmid Preparation:

    Article Title: Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2
    Article Snippet: .. ANO2 expressing FRT cells were obtained by stable transfection of FRT cells with a pCMV6-ANO2 (Origene Technologies Inc), plasmid which expresses the mouse ANO2 gene and a pcDNA3.1-YFP-F46L/H148Q/I152L plasmid which expresses halide sensor YFP gene. ..



    Similar Products

    mouse tmem16b (hugo-approved human gene symbol ano2; mim# 610109; (cherian, menini, & boccaccio, 2015; pifferi, dibattista, & menini, 2009)  (imaGenes GmbH)
    90
    imaGenes GmbH mouse tmem16b (hugo-approved human gene symbol ano2; mim# 610109; (cherian, menini, & boccaccio, 2015; pifferi, dibattista, & menini, 2009)
    Mouse Tmem16b (Hugo Approved Human Gene Symbol Ano2; Mim# 610109; (Cherian, Menini, & Boccaccio, 2015; Pifferi, Dibattista, & Menini, 2009), supplied by imaGenes GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ano2+gene/cdna+for+mouse+tmem16b/pm32112655-35-4-26
    Average 90 stars, based on 1 article reviews
    mouse tmem16b (hugo-approved human gene symbol ano2; mim# 610109; (cherian, menini, & boccaccio, 2015; pifferi, dibattista, & menini, 2009) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse ano2 gene  (OriGene)
    90
    OriGene mouse ano2 gene
    A-C) YFP Fluorescence measurement showing inhibition of mouse <t>ANO2</t> activity by Ani9, T16A inh -A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).
    Mouse Ano2 Gene, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ano2+gene/Ano2+Mouse+qPCR+Template+Standard/pmc04878759-34-22-15
    Average 90 stars, based on 1 article reviews
    mouse ano2 gene - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A-C) YFP Fluorescence measurement showing inhibition of mouse ANO2 activity by Ani9, T16A inh -A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).

    Journal: PLoS ONE

    Article Title: Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2

    doi: 10.1371/journal.pone.0155771

    Figure Lengend Snippet: A-C) YFP Fluorescence measurement showing inhibition of mouse ANO2 activity by Ani9, T16A inh -A01 and MONNA in FRT cells expressing ANO2 and a halide sensor YFP. The indicated concentrations of the inhibitors were applied 20 min prior to ANO2 activation by 100 μM ATP. D) Apical membrane current was measured in FRT-ANO1cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO1 activation. E) Apical membrane current was measured in FRT-ANO2 cells. 1 μM Ani9, 10 μM T16A inh -A01 and 10 μM MONNA were applied 20 min prior to ANO2 activation. F) Summary of peak currents of ANO1 and ANO2 (mean ± S.E., n = 4). G) Representative current traces showing Ani9-induced ANO2 inhibition at the indicated concentration. H) Summary of dose responses of ANO1 and ANO2 (mean ± S.E., n = 4–6).

    Article Snippet: ANO2 expressing FRT cells were obtained by stable transfection of FRT cells with a pCMV6-ANO2 (Origene Technologies Inc), plasmid which expresses the mouse ANO2 gene and a pcDNA3.1-YFP-F46L/H148Q/I152L plasmid which expresses halide sensor YFP gene.

    Techniques: Fluorescence, Inhibition, Activity Assay, Expressing, Activation Assay, Concentration Assay